Journal: Cell reports

Article Title: Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networks

doi: 10.1016/j.celrep.2026.117027

Figure Lengend Snippet: (A) Strategy to deliver pCAGIG plasmids into neocortices via IUE of E14.5 Emx1- Upf2 cKO cortices and their littermate controls. (B) Immunostaining of GFP and TBR1 in E17.5 Emx1- Upf2 cKO cortices and their littermate controls. Scale bars, 50 μm. (C) Immunostaining of GFP and TBR2 in E17.5 Emx1- Upf2 cKO cortices and their littermate controls. Scale bars, 50 μm. (D) Images of GFP + cells in E18.5 Emx1- Upf2 cKO cortices and their littermate controls. Scale bars, 50 μm. (E) Quantification of percentages of GFP + cells per bin. The E18.5 neocortices from basal to apical surfaces were divided into 8 equal bins as shown in (D) ( n = 2; N = 2 sections per group and 10 images per section; data are represented as the mean ± SD; * p < 0.05 and ** p < 0.01; paired t test). (F) Schematic of the Double UP plasmid, co-electroporated with Cre plasmid into Upf2 fl/fl embryos at E13.5. (G) Left: representative images of Upf2 fl/fl cortices electroporated with Double UP plasmid at E13.5 and analyzed at E17.5 (scale bars, 50 μm). Right: quantification of cell distribution across ten bins from the pial to ventricular surface. The p values were calculated using t test corrected by the Holm-Sidak method (α = 0.05) without assuming a consistent standard deviation. Error bars represent SEM.

Article Snippet: For layer marker analysis experiments, Upf2 fl/+ ;Emx1 + male breeders were crossed with Upf2 fl/fl females and electroporated at E14.5 with pCAGIG (Addgene#11159) plasmid and collected at E16.5-E18.5.

Techniques: Immunostaining, Plasmid Preparation, Standard Deviation

Journal: Cell reports

Article Title: Nonsense-mediated mRNA decay orchestrates neuronal migration and cortical lamination while modulating Reelin and ciliary gene regulatory networks

doi: 10.1016/j.celrep.2026.117027

Figure Lengend Snippet: (A) Schematic of the Double UP plasmid construct encoding FLAG-tagged FOXJ1. (B) Quantification of migration distances of sfGFP + mScarlet − and mScarlet + neurons from the ventricular surface. Data were pooled across all biological replicates. The p value was calculated using Welch’s t test. (C) Mean migration distance of sfGFP + mScarlet − and mScarlet + groups for each biological replicate. The p value was calculated using paired t test. (D) Left: representative images of brains electroporated with FLAG-FOXJ1 Double UP plasmid at E14.5 and analyzed at E17.5 (scale bars, 50 μm). Right: quantification of cell distribution across ten equally divided bins from the pial to ventricular surface. The p values were calculated using t test corrected by the Holm-Sidak method (α = 0.05) without assuming a consistent standard deviation. Error bars represent SEM.

Article Snippet: For layer marker analysis experiments, Upf2 fl/+ ;Emx1 + male breeders were crossed with Upf2 fl/fl females and electroporated at E14.5 with pCAGIG (Addgene#11159) plasmid and collected at E16.5-E18.5.

Techniques: Plasmid Preparation, Construct, Migration, Standard Deviation